RTG-ReMPro
Projects
P7 Damme
Functional and regulatory effect of posttranslational modifications
on the frontotemporal lobar degeneration risk factor TMEM106B
Objectives
Our major goal is to obtain a better understanding of the relevance and function of posttranslational modifications of TMEM106 B. We hypothesize that the posttranslational modifications modulate the sorting of TMEM106 B from the Golgi apparatus to lysosomes, the interaction with other proteins, and (presumably) indirectly, the formation of TMEM106 B fibrils.
Aim i) Full characterization of PTMs of TMEM106 B
Aim ii) Effect of PTMs on intracellular sorting
Aim iii) Effect of PTMs of fibril-formation
Aim iv) Effect of PTMs on homo- / and heterodimerization with TMEM106 B /C
Aim v) Characterization of the AID PROTAC system for TMEM106 B
Topology and structure of TMEM106B in the lysosomal membrane. Posttranslational modifications such as N-myristoylation and phosphorylation in the cytoplasmic N-terminus are depicted.
